Sensitive and validated TLC densitometry method coupled with fluorescence detection for quantitative determination of the newly co-formulated drugs, celecoxib and amlodipine besylate in tablet dosage form

Abstract New, sensitive, rapid, cost-effective, and validated stability-indicating thin layer chromatographic (TLC) method coupled with fluorescence (FL) detection was developed for the quantitative analysis of celecoxib (CEL) amlodipine besylate (AMLO) in their laboratory prepared binary mixture using non-fluorescent TLC silica gel 60 plates. Ethyl acetate: diethylamine: 1-propanol (9:1:0.2, V/V) used as a developing system. The retention factor (R f ) each drug 0.80 ± 0.03 0.44 0.01 CEL AMLO, respectively. plates were excited at 264 nm simultaneous FL measurement calibration curves linear over concentration ranges 30.0–300.0 ng/band 15.0–150.0 mean percentage recoveries 99.80 0.85 0.77 For applied stability studies cited drugs forced degradation products determined when present presence pure so can be considered one it per ICH guidelines proved to accurate precise.